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http://ri.uaemex.mx/handle20.500.11799/65536| Title: | Thermotherapy Research Subject Categories Potato virus X (PVX) elimination as short and long term effects of hydrogen peroxide and salicylic acid is differentially mediated by oxidative stress in synergism with thermotherapy |
Keywords: | Reactive oxygen species;info:eu-repo/classification/cti/2 | Publisher: | Springer | Description: | Articulo científico indezado Abstract Short and long term effects of hydrogen peroxide (H2O2) and salicylic acid (SA) were examined in: thermotolerance, virus X (PVX)-free microplants obtained by in vitro thermotherapy, catalase activity (CAT) and H2O2 concentration. Short term effects (STE) were tested as follows: (a) nodal explants were cultivated 30 d on MS medium containing SA 10−5 or 10−6 M; and (b) nodal explants were waterlogged for 1 h in 1 or 5 mMH2O2 solution and subsequently cultured in MS for 30 d. Long term effects were tested as follows (LTE): the experimental regime was identical to STE but SA and H2O2 treated plants were subcultured for an additional 30 d period onMS. All treatments were followed by thermotherapy (32–42 C) for 35 d. Results showed SA and H2O2 induced thermotolerance during thermotherapy. The percentage of PVX-free plants obtained in H2O2 was significantly higher than in SA in STE and LTE by 3-and 4 fold respectively. CAT activity was differentially mediated by SA and H2O2. This research was supported by a grant from Recursos Fiscales, INIFAP. |
URI: | http://ri.uaemex.mx/handle20.500.11799/65536 | Other Identifiers: | http://hdl.handle.net/20.500.11799/65536 | Rights: | info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0 |
| Appears in Collections: | Producción |
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