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http://ri.uaemex.mx/handle20.500.11799/66027| Title: | Influence of individual or mixed cellulase and xylanase mixture on in vitro rumen gas production kinetics of total mixed rations with different maize silage and concentrate ratios | Authors: | MONA MOHAMED MOHAMED YASSEEN ELGHANDOUR AHMED Eid OFELIA MARQUEZ MOLINA José Fernando Vázquez Armijo Anil Kumar Abdelfattah Zeidan Mohamed Salem |
Keywords: | Cellulase;fibrolytic enzymes;maize silage;concentrate ratio;xylanase;info:eu-repo/classification/cti/6 | Publisher: | Turkish Journal of Veterinary and Animal Sciences | Project: | Vol.;39 | Description: | Cellulase, xylanase, and β-D glucanase enzymes are specific for the breaking of internal β-1,4 linkages of cellulose, hemicellulose (xylan), and glucans to release soluble sugars and facilitate the growth of microbes, thereby increasing the in vitro dry matter (DM) digestibility (8). Morgavi et al. (7) demonstrated the synergism between exogenous and endogenous rumen enzymes such that the net combined hydrolytic effect in the rumen was much higher than that estimated from individual enzyme activities. Exogenous enzymes may be applied during ensiling or directly fed to animals during feeding (1). Enzyme application during ensiling gives an economical benefit to farmers by increasing the feed intake (i.e. palatability) and digestion rate (1,9). Direct addition of enzymes to animal feeds just before feeding is much easier and more applicable in terms of agronomic practices. This study assessed the effects of cellulase, xylanase, and their mixture (1:1, v/v) on in vitro rumen fermentation of five mixed rations with different maize silage (F) to concentrate (C) ratios (0F:100C, 25F:75C, 50F:50C, 75F:25C, 100F:0C). Samples were incubated using rumen inoculum from Brown Swiss cows fed ad libitum a total mixed ration of concentrate and alfalfa hay (1:1). Gas production (GP) was recorded after 2, 4, 6, 8, 10, 12, 24, 36, 48, and 72 h of incubation. Interaction effects (P < 0.0001) were observed between type of ration and enzyme for discrete lag time prior to GP. Ration type affected asymptotic GP (linear and quadratic, P < 0.0001) and in vitro GP at 24 h onward. Moreover, rations affected pH (linear and quadratic, P < 0.0001), dry matter degradability (quadratic, P = 0.05), metabolizable energy (linear, P = 0.038), short-chain fatty acids (linear, P = 0.0005), gas yield from truly digested dry matter at 24 h (linear, P = 0.0026), and microbial crude protein yield (linear, P = 0.0264). The most effective rations for in vitro GP during different times differed between different measured parameters. Enzyme administration was more effective when the ratio of maize silage was increased. |
URI: | http://ri.uaemex.mx/handle20.500.11799/66027 | Other Identifiers: | http://hdl.handle.net/20.500.11799/66027 | Rights: | info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0 |
| Appears in Collections: | Producción |
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