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http://ri.uaemex.mx/handle20.500.11799/67425| Title: | Biodegradable poly(D,L-lactide-co-glycolide)/poly(L-γ-glutamic acid) nanoparticles conjugated to folic acid for targeted delivery of doxorubicin | Keywords: | Folic acid;PLGA nanoparticles;Targeted drug delivery;Multimeric FA nanoparticles;Sustained-release system;info:eu-repo/classification/cti/2 | Publisher: | Elsevier, Materials Science and Engineering C | Project: | DOI;http://dx.doi.org/10.1016/j.msec.2017.03.145 | Description: | A novel targeted drug delivery nanoparticle system based on poly(D,L-lactide-co-glycolide) acid (PLGA) for delivery of doxorubicin (DOX) was developed. DOX-PLGA NPs were obtained by the emulsification-solvent evaporation technique. Then, their surface was modified with poly(L-γ-glutamic acid) (γ-PGA) and finally conjugated to modified folic acid (FA) as a targeting ligand. The surface modification and FA conjugation were followed by UV–Vis and FT-IR spectroscopies. Morphology was observed by TEM/SEM. Particle size, PDI and zeta potential were measured using DLS studies. Encapsulation and loading efficiencies, and DOX release kinetics were determined. Specific uptake and cell viability of DOX-PLGA/γ-PGA-FA NPs were tested in HeLa cells. Quasi-spherical nanoparticleswith a particle size lower than 600nm(DLS)were obtained. Spectroscopic techniques demonstrated the successful surface modification with γ-PGA and FA conjugation. Release profile of DOX-PLGA/γ-PGA-FA NPs showed a release of 55.4 ± 0.6% after seven days, in an acidic environment. HeLa cells exhibited a decrease in viability when treated with DOX-PLGA/γ-PGA-AF NPs, and cellular uptake was attributed to FA receptor-mediated endocytosis. These results suggest that DOX-PLGA/γ-PGA-FA NPs are a potential targeted drug carrier for further applications in cancer therapy. This study was supported by the International Atomic Energy Agency (CRP-F22064, Contract No. 18358) and the Universidad Autónoma del Estado de México, through the project No. 3543/2013CHT. |
URI: | http://ri.uaemex.mx/handle20.500.11799/67425 | Other Identifiers: | http://hdl.handle.net/20.500.11799/67425 | Rights: | info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0 |
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