Enzymatic preparation of structured triacylglycerides containing γ-linolenic acid

Please use this identifier to cite or link to this item: http://ri.uaemex.mx/handle20.500.11799/109460

Title:	Enzymatic preparation of structured triacylglycerides containing γ-linolenic acid
Keywords:	GLA;Lipases;Solvent-free;Structured triglycerides;info:eu-repo/classification/cti/7
Publisher:	Biocatalysis and Agricultural Biotechnology
Project:	28
Description:	with the addition of molecular sieves (MS) at different addition times (3, 9, 12 and 15 h) and loads (2.5, 5, 10 and 15%, based on the total weight of substrates), the enrichment of GLA from evening primrose oil (EPO) and 1- butanol (BtOH) was improved via Candida rugosa lipase-catalysed esterification reactions. Secondly, the GLAenriched fraction was separated by thin-layer chromatography (TLC) to be further set to react during the third step in the presence of glycerol and Candida antarctica fraction B (CALB), under different enzyme loadings (5, 10, 15 and 20%, based on the total weight of substrates), temperatures (30, 40, 50 and 60 ◦C) and substrates molar ratios (1:1, 2:1, 3:1 and 4:1, GLA:glycerol). 60% of STAG containing 49 wt% of GLA were produced by using 15% of CALB at 60 ◦C and a 3:1 molar ratio. Conacyt: Beca no. 328716 UAEMex: Proyecto 3866/2015/PIC
URI:	http://ri.uaemex.mx/handle20.500.11799/109460
Other Identifiers:	http://hdl.handle.net/20.500.11799/109460
Rights:	info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0
Appears in Collections:	Producción

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