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http://ri.uaemex.mx/handle20.500.11799/67422| Title: | Preparation and characterization of a tumor-targeting dual-image system based on iron oxide nanoparticles functionalized with folic acid and rhodamine | Authors: | ALEJANDRA ANCIRA CORTEZ ENRIQUE MORALES AVILA BLANCA ELI OCAMPO GARCIA CARLOS GONZALEZ ROMERO LUIS ALBERTO MEDINA VELAZQUEZ GUSTAVO LOPEZ TELLEZ Erick Cuevas Yáñez |
Keywords: | Tumor-Targeting Dual-Image System Based;Iron Oxide Nanoparticles;Folic Acid;Nanotechnology;info:eu-repo/classification/cti/2 | Publisher: | Hindawi | Project: | doi;https://doi.org/10.1155/2017/5184167 Vol;2017 ID;5184167 |
Description: | Cancer is one of the diseases with most deaths worldwide, around 8.2 million annually. For this reason, several treatments and diagnostic tools have been investigated and developed over the past decades. Among them, a dual-image system has been developed to achieve and enhance the detection of cancer, which has not been done with systems currently available. The present study describes the preparation of a dual-image targeting system composed of magnetic iron oxide nanoparticles functionalized with folic acid and rhodamine; nanoparticles synthesis was achieved by a coprecipitation method; the functionalization was carried out by a carbodiimide with folic acid and/or the rhodamine isothiocyanate; conjugates were characterized by spectrometric techniques; toxicity was measured by cell proliferation assay on HeLa cells using progressive concentrations of functionalized nanoparticles. Cellular uptake assay was carried out by competitive assay on HeLa cells. Iron oxide magnetite nanoparticles, modified with folic acid and rhodamine, were successfully synthetized with a particle size lower than 20nm (TEM), EDS, HRTEM, and XDR showed highly crystalline Fe3O4 nanoparticles. Folic acid and rhodamine were conjugated with high efficiency. A significant selectivity and uptake, facilitated by surface modification of iron oxide nanoparticles with folic acid, were demonstrated.The multifunctional system showed suitable physicochemical and biological properties for cell targeting through folate receptors. This study was supported by the International Atomic Energy Agency (CRP-F22064, Contract no. 18358) and the Universidad Autónoma del Estado de México, through Project no. 3543/2013CHT. |
URI: | http://ri.uaemex.mx/handle20.500.11799/67422 | Other Identifiers: | http://hdl.handle.net/20.500.11799/67422 | Rights: | info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0 |
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