Influence of exogenous enzymes in presence of Salix babylonica extract on digestibility, microbial protein synthesis and performance of lambs fed maize silage

Abstract

Sixteen Suffolk lambs with 29 ± 2·0 kg body weight were housed in individual cages for 60 days and allotted to four treatments in a completely randomized design to determine the effect of administration of Salix babylonica (SB) extract and/or exogenous enzymes (ZADO®) on lamb performance. Lambs were fed with 300 g/kg concentrate (160 g crude protein (CP)/kg, 13·4 MJ metabolizable energy (ME)/kg dry matter (DM)) and 700 g/kg maize silage (80 g/kg CP, 11·7 MJ ME/kg DM) as a basal diet (control). Another three treatments were tested; the SB extract was administered at 30 ml/day (SB) and exogenous enzymes ZADO® (i.e. an exogenous enzyme cocktail in a powder form) directly fed at 10 g/day (EZ), while the last treatment contained ZADO® at 10 g/day + SB extract at 30 ml/day (EZSB). Lambs of the treatment EZSB had the greatest average daily weight gain (ADG) and feed conversion throughout the period of the experiment. However, during the first 30 days SB was more effective for ADG than EZ and vice versa during the last 30 days of the experiment.Water consumption was greater for SB, followed by EZ and EZSB compared to the control. Intakes ofDMand organicmatter (OM) were the highest in EZSB followed by EZ, which had the greatest neutral detergent fibre, acid detergent fibre (ADF) and nitrogen (N) intakes. The EZSB treatment had the greatest DM and OM digestibilities compared to the other treatments; however, SB had the greatest ADF digestibility. Combination of EZ and SB had the best N balance. Allantoin, total purine derivatives (PD), allantoin : -creatinine ratio, and PD: creatinine ratio were increased in EZSB compared to the other treatments. However, EZ supplementation increased uric acid concentration, whereas the microbial N (g N/day) and metabolizable protein (g N/day) were increased in EZSB versus the other treatments. It can be concluded that addition of 10 g ZADO® in combination with S. babylonica extract at 30 ml/day in the diet of lambs increased feed intake, nutrient digestibility and daily gain, with a positive impact on the use of N and microbial protein synthesis.