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dc.contributor.author Arzate-Fernández, Amaury-Martín
dc.contributor.author Reyes-Díaz, Jesús-Ignacio
dc.contributor.author Piña-Escutia, José-Luis
dc.contributor.author Vázquez-García, Luis-Miguel
dc.date.accessioned 2018-02-14T19:07:04Z
dc.date.available 2018-02-14T19:07:04Z
dc.date.issued 2017-06-21
dc.identifier.issn 0926-6690
dc.identifier.uri http://hdl.handle.net/20.500.11799/68585
dc.description.abstract A complete protocol of somatic embryogenesis involves induction of embryogenic callus, embryo development, embryo maturation, and their conversion or germination to form complete plants; in this sense judicious se- lection of nutrient medium, growth regulators, and physical culture environment is required. In this work, culture medium factors that influence somatic embryogenesis in Agave angustifolia were investigated. In an induction medium (IM) we evaluated the effect of three sucrose concentrations, three plant growth regulator (PGR) combinations, two groups of vitamins and two sources of amino acids. We observed that somatic embryos (SE) in medium containing 6% sucrose concentration grew vigorously, while those induced in medium with 8% sucrose were abnormally shaped and did not develop fully. In contrast, a higher sucrose concentration (10%) inhibited development of explants. Embryogenic callus cultured in IM containing 13.59μM 2,4-di- chlorophenoxyacetic acid (2,4-D) and 4.44 μM 6-benzyladenine (BA) only produced SE, while those explants assayed in IM with 11.34 μM abscisic acid (ABA) did not improve embryogenesis response. Phillips-Collins (L2) vitamins induced a higher number of SE (34 ± 0.4) than Murashige–Skoog (MS) vitamins (1.7 ± 0.7). High levels of amino acids (500 mg l−1 L-glutamine or casein hydrolysate) were not effective in promoting embryogenesis. Conversion frequency to plantlets ranged from 95 to 100% with 100% survival under ex vitro conditions. es
dc.language.iso eng es
dc.publisher Industrial Crops and Products Journal es
dc.relation.ispartofseries http://dx.doi.org/10.1016/j.indcrop.2017.06.021;
dc.rights embargoedAccess es
dc.rights https://creativecommons.org/licenses/by-nc-nd/4.0/ es
dc.rights embargoedAccess es
dc.rights https://creativecommons.org/licenses/by-nc-nd/4.0/ es
dc.subject Media cuture es
dc.subject Somatic embryogenesis es
dc.subject Agave angustifolia es
dc.title Media culture factors affecting somatic embryogenesis in Agave angustifolia Haw. es
dc.type Artículo es
dc.provenance Científica es
dc.road Dorada es
dc.organismo Ciencias Agrícolas es
dc.ambito Internacional es
dc.cve.CenCos 21301 es


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  • Título
  • Media culture factors affecting somatic embryogenesis in Agave angustifolia Haw.
  • Autor
  • Arzate-Fernández, Amaury-Martín
  • Reyes-Díaz, Jesús-Ignacio
  • Piña-Escutia, José-Luis
  • Vázquez-García, Luis-Miguel
  • Fecha de publicación
  • 2017-06-21
  • Editor
  • Industrial Crops and Products Journal
  • Tipo de documento
  • Artículo
  • Palabras clave
  • Media cuture
  • Somatic embryogenesis
  • Agave angustifolia
  • Los documentos depositados en el Repositorio Institucional de la Universidad Autónoma del Estado de México se encuentran a disposición en Acceso Abierto bajo la licencia Creative Commons: Atribución-NoComercial-SinDerivar 4.0 Internacional (CC BY-NC-ND 4.0)

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