Estimation of anthropogenic organo-chlorine, bromine and iodine compounds in apolar lipid fractions of bovine milk by solid-phase extraction and neutron activation analysis (SPE–NAA)

Abstract

Milk lipids were separated using a hexane:isopropanol mixture; then they were fractionated into apolar, medium polar and polar portions by SPE using a LC-Si column. The apolar fraction was further separated into 4 more portions depending on their polarity by another SPE method using a Florosil column. Levels of Cl, Br and I were measured by NAA. Halogens were detected in all separated fractions; but their highest levels were found in the most apolar fraction containing hydrocarbons which strongly indicates the presence of anthropogenic organohalogen compounds in milk. In addition, iodide and iodate ions in whole milk samples were measured.