Resumen:
The desideratum aim of the present context was to isolate a promising antagonist probiotic bacterium from
fermented food item as biocontrol agent against uropathogens. Among diversified isolates evaluated for antagonistic
trait, Staphylococcus succinus strain AAS2 was found to be an auspicious candidate against urinary tract
infection (UTI) causing bacterial pathogens, being the most active against Staphylococcus aureus with substantial
activity of 352.5 ± 5.4 AU/mL. Further, the in vitro probiotic attributes of strain AAS2 were assessed using
systematic methodology. The isolate exhibited tolerance to acidic condition (up to pH 3.0) and simulated gastric
juice (at pH 3.0) with fairly high survival logarithmic cell counts of 5.3 ± 0.15 and 5.23 ± 0.02 log cfu/mL,
respectively. Additionally, strain AAS2 showed capability to resist 0.5% w/v bile salt too. It also revealed significant
values of auto-aggregation (32.5 ± 1.3–56.5 ± 1.4%) and cell surface hydrophobicity
(38.35 ± 1.4%) properties. The isolate showed resistivity towards phenol (6.8 ± 0.08 log cfu/mL) and lysozyme
(58.6 ± 1.6%). Further, the susceptibility trait of strain AAS2 to conventional antibiotics made this isolate
a promising probiotic bacterium. Most importantly, the isolate depicted DPPH (2,2-Diphenyl-1-picrylhydrazyl)
and hydroxyl radical scavenging activities in a concentration dependent manner, thereby exhibiting its propitious
antioxidative properties. In a nutshell, the outcomes of this investigation divulge the plausible use of S.
succinus strain AAS2 as biocontrol agent against uropathogens, and recommended further applications in
pharmaceutics due to its pronounced probiotic traits.