The potential of rumen fluid waste from slaughterhouses as an environmentally friendly source of enzyme additives for ruminant feedstuffs

Abstract

Rumen fluid disposal from slaughterhouses represents a major environmental challenge due to the presence of high levels of ammonia and phosphorus. The loading of these limiting nutrients into the soil and aquatic systems triggers eutrophication. Fortunately, the fluid is also rich in fibrolytic and other enzymes that could, alternatively, be used to enhance feed utilization in animals. Therefore, the aim of this study was to evaluate rumen fluid as a potential source of exogenous feed enzymes using a variety of test substrates. Hydrolytic enzyme activities of carboxymethyl cellulase, a-amylase and microcrystalline cellulase (avicellase) were measured in the rumen fluid to determine its enzymatic capabilities. Centrifuged and sonicated rumen fluid was used to pre-treat milled corn grain, barley grain, soybean meal, common vetch grain, bitter vetch grain, chickling vetch grain, alfalfa hay and common vetch straw substrates at 0, 1, 2, 3, or 4 mL per 100 g dry matter. Rumen fluid-treated substrates were subjected to a water solubility test and in vitro ruminal fermentation. The activities of carboxymethyl cellulase, avicellase and amylase were observed to be 377.8, 333.4 and 282 U/mL, respectively. Water solubility of dry matter in bitter vetch grain, chickling vetch grain, common vetch grain and soybean meal increased linearly with level of rumen fluid treatment. The highest solubility was observed in substrates treated with 4 mL rumen fluid per 100 g dry matter (P < 0.05). With the exception of common vetch grain, other feeds had the highest (P < 0.05) biogas production at 24 and 48 h when pre-treated with 3 and 4 mL rumen fluid per 100 g dry matter. Pre-treatment of feeds with rumen fluid significantly (P < 0.05) improved total fermentable fraction of corn grain, bitter vetch grain, chickling vetch grain, alfalfa hay and common vetch straw. However, digestible organic matter and metabolizable energy of common vetch grain were not influenced by rumen fluid pre-treatment. These results showed that rumen fluid has the potential to be used as an environmentally friendly source of exogenous feed enzymes that enhance feed utilization in ruminants.