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dc.contributor.author	MARTINEZ LUNA, GRISET
dc.contributor.author	CASTILLO CADENA, JULIETA
dc.contributor.author	SERMENT GUERRERO, JORGE HUMBERTO
dc.creator	MARTINEZ LUNA, GRISET; 413531
dc.creator	CASTILLO CADENA, JULIETA; 202796
dc.creator	SERMENT GUERRERO, JORGE HUMBERTO; 169664
dc.date.accessioned	2016-03-16T17:16:43Z
dc.date.available	2016-03-16T17:16:43Z
dc.date.issued	2015
dc.identifier	http://www.redalyc.org/articulo.oa?id=37036860003
dc.identifier.uri	http://hdl.handle.net/20.500.11799/39050
dc.description	The comet assay is a relatively inexpensive, fast, sensitive and reliable method to detect DNA breakage upon individual cells. The most widely used cell type in this technique is lymphocyte, because they are easy to obtain and handle, and because they are continually exposed to xenobiotics that enter into the body. However, is important to consider the possibility to use other cell types for very specific purposes. Sperm cells are of special interest because they could be used as a biomonitor to risk assessment in populations occupationally exposed to xenobiotics. Besides, the fact that there are not functional DNA repair mechanisms in these cells could increase the sensitivity of the system. We present here several modifications to the comet assay methodology to evaluate DNA breakage in sperm cells with reliable results.
dc.format	application/pdf
dc.language.iso	eng	es
dc.publisher	Universidad Nacional Autónoma de México
dc.relation	http://www.redalyc.org/revista.oa?id=370
dc.rights	Revista Internacional de Contaminación Ambiental
dc.rights	openAccess	es
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0
dc.source	Revista Internacional de Contaminación Ambiental (México) Num.1 Vol.31
dc.subject	Ciencias de la Tierra	es
dc.subject	Genotoxicity	es
dc.subject	DNA breakage	es
dc.subject	sperm	es
dc.subject	microelectrophoresis	es
dc.subject.classification	CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA
dc.title	Modified procedure to assess dna breakage in spermatozoa by means of the comet assay	es
dc.type	Artículo	es
dc.provenance	Científica
dc.road	Dorada	es
dc.ambito	Internacional	es
dc.audience	students	es
dc.audience	researchers	es
dc.type.conacyt	article
dc.identificator	1

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