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dc.contributor.author	LOPEZ HEYDECK, SANDRA MARICRUZ
dc.contributor.author	Cajero Juárez, Marcos
dc.contributor.author	ALONSO MORALES, ROGELIO ALEJANDRO
dc.contributor.author	MARTINEZ CASTAÑEDA, JOSE SIMON
dc.contributor.author	Robles González, Jose Francisco
dc.contributor.author	BARBABOSA PLIEGO, ALBERTO
dc.contributor.author	VAZQUEZ CHAGOYAN, JUAN CARLOS
dc.creator	LOPEZ HEYDECK, SANDRA MARICRUZ; 889387
dc.creator	Cajero Juárez, Marcos; 31050
dc.creator	ALONSO MORALES, ROGELIO ALEJANDRO; 14610
dc.creator	MARTINEZ CASTAÑEDA, JOSE SIMON; 30838
dc.creator	Robles González, Jose Francisco;x1256063
dc.creator	BARBABOSA PLIEGO, ALBERTO; 348264
dc.creator	VAZQUEZ CHAGOYAN, JUAN CARLOS; 120705
dc.date.accessioned	2016-03-16T17:17:09Z
dc.date.available	2016-03-16T17:17:09Z
dc.date.issued	2009
dc.identifier	http://www.redalyc.org/articulo.oa?id=42311566009
dc.identifier.uri	http://hdl.handle.net/20.500.11799/39212
dc.description	Electroporation has been the method of election for transfection of murine embryonic stem cells for over 15 years; however, it is a time consuming protocol because it requires large amounts of DNA and cells, as well as expensive and delicate equipment. Lipofection is a transfection method that requires lower amounts of cells and DNA than electroporation, and has proven to be effi cient in a large number of cell lines. It has been shown that after lipofection, mouse embryonic stem cells remain pluripotent, capable of forming germ line chimeras and can be transfected with greater effi ciency than with electroporation; however, gene targeting of mouse embryonic stem cells by lipofection has not been reported. The objective of this work was to fi nd out if lipofection can be used as effi ciently as electroporation for regular gene targeting protocols. This context compares gene targeting effi ciency between these techniques in mouse embryonic stem cells E14TG2a, using a gene replacement type vector. No differences were found in gene targeting effi ciency between groups; however, lipofection was three times more effi cient than electroporation in transfection effi ciency, which makes lipofection a less expensive alternative method to produce gene targeting in mouse embryonic stem cells.
dc.format	application/pdf
dc.language.iso	eng	es
dc.publisher	Universidad Nacional Autónoma de México
dc.relation	http://www.redalyc.org/revista.oa?id=423
dc.rights	Veterinaria México
dc.rights	openAccess	es
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0
dc.source	Veterinaria México (México) Num.1 Vol.40
dc.subject	veterinaria	es
dc.subject	gene targeting	es
dc.subject	homologous recombination	es
dc.subject	mouse embryonic stem cells	es
dc.subject	transfection	es
dc.subject	electroporation	es
dc.subject	lipofection	es
dc.subject.classification	CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA
dc.title	Lipofection improves gene targeting efficiency in E14 TG2a mouse embryonic stem cells	es
dc.type	Artículo	es
dc.provenance	Científica
dc.road	Dorada	es
dc.ambito	Internacional	es
dc.audience	students	es
dc.audience	researchers	es
dc.type.conacyt	article
dc.identificator	6

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