The immunogenic protection response to four P. multocida isolations obtained from clinical cases and a reference strain was studied. Isolations were proven as three different immunogens: complete antigen (Ag), washed Ag and culture supernatant. They were subcutaneously administered in SPF light White Leghorn hens. Immune response was evaluated using ELISA test and challenge test evaluated protection response. The ANOVA and Tukey test did not show statistical differences between groups. All isolations using different vaccines induced high protection levels ranging from 87 to 100%. This study indicates that immunization using these three immunogens induce an effective response against P. multocida challenge with the best protection when culture supernatant is used.