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dc.contributor.author Donald Arguedas Cortés
dc.contributor.author Alex P. Romero Zuñiga
dc.contributor.author Ricardo Enriquez Sais
dc.contributor.author José S. Martínez Castañeda
dc.contributor.author César Ortega Santana
dc.creator Donald Arguedas Cortés
dc.creator Alex P. Romero Zuñiga
dc.creator Ricardo Enriquez Sais
dc.creator José S. Martínez Castañeda
dc.creator César Ortega Santana
dc.date 2015
dc.date.accessioned 2016-03-16T17:17:19Z
dc.date.available 2016-03-16T17:17:19Z
dc.identifier http://www.redalyc.org/articulo.oa?id=44938603018
dc.identifier.uri http://hdl.handle.net/20.500.11799/39268
dc.description The infectious pancreatic necrosis (IPNV) is the causative agent of an acute illness well characterized in salmonids worldwide. Clinical signs and mortality rates are dependent on several factors such as the viral dose, the age of the fish, the water temperature, among others. An experimental study was conducted to measure the effect of temperature on the gene expression profile of IFN-1(α), STAT-1 and Mx-1 in rainbow trout fry, exposed to IPNV. Fry (n=198) were exposed at 8, 12 and 16°C, and samples were taken for 21 days to determine the virus titer and gene expression. In the first 11 days the greatest viral titer was recorded at 8°C compared with the values obtained at 12 and 16°C. At 8°C, there was a significant increase on day 4 of mRNA Mx-1 (t-test, p<0.05), time in which the viral titer began to decrease. Furthermore, as the viral titer increased, STAT-1 and Mx-1 (r=0.91) and (r=0.96) increased, respectively. The animals were able to recover from day 4 from some of the symptoms of IPN. Clinical disease was developed only in fish exposed to 12°C and all died between days 6 and 14, despite the highly significant increase shown in the average expression level of Mx-1, compared with the values recorded at 8°C and 16°C (Tukey, p<0.0001). Additionally, the expression profiles of IFN-1(α) and STAT-1 decreased completely (~0.016) and (~0.020 times) on day 7. The highest expression level of IFN-1(α), occurred at 16°C (Tukey, p<0.0005). Fry exposed at 16°C were normal during the experiment. IFN-1(α) possibly generated a protector effect from day 2 when they showed a significant expression increase compared with the results at 8°C and 12°C (t-student, p<0.0001); however, STAT-1 was not significantly affected by temperature, although the highest average expression value was recorded at 16°C. Our research supports the expression of relevant anti-viral response genes as IFN-1(α), STAT-1 and Mx-1 are physiologically modulated by the water temperature, directly influencing the development of the IPN disease in rainbow trout. Rev. Biol. Trop. 63 (2): 559-569. Epub 2015 June 01.
dc.format application/pdf
dc.language eng
dc.publisher Universidad de Costa Rica
dc.relation http://www.redalyc.org/revista.oa?id=449
dc.rights openAcess
dc.rights Revista de Biología Tropical
dc.source Revista de Biología Tropical (Costa Rica) Num.2 Vol.63
dc.subject Biología
dc.subject IPNV
dc.subject temperature
dc.subject rainbow trout
dc.subject fry
dc.subject ISGs
dc.subject real-time PCR
dc.subject mortality
dc.title Effect of temperature on the expression of IFN-1 (α), STAT-1 and Mx-1 genes in Oncorhynchus mykiss (Salmoniformes: Salmonidae) exposed with the virus of the infectious pancreatic necrosis (IPNV)
dc.type Artículo
dc.provenance Científica
dc.road Dorada


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  • Título
  • Effect of temperature on the expression of IFN-1 (α), STAT-1 and Mx-1 genes in Oncorhynchus mykiss (Salmoniformes: Salmonidae) exposed with the virus of the infectious pancreatic necrosis (IPNV)
  • Autor
  • Donald Arguedas Cortés
  • Alex P. Romero Zuñiga
  • Ricardo Enriquez Sais
  • José S. Martínez Castañeda
  • César Ortega Santana
  • Editor
  • Universidad de Costa Rica
  • Tipo de documento
  • Artículo
  • Palabras clave
  • Biología
  • IPNV
  • temperature
  • rainbow trout
  • fry
  • ISGs
  • real-time PCR
  • mortality
  • Los documentos depositados en el Repositorio Institucional de la Universidad Autónoma del Estado de México se encuentran a disposición en Acceso Abierto bajo la licencia Creative Commons: Atribución-NoComercial-SinDerivar 4.0 Internacional (CC BY-NC-ND 4.0)

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