Mostrar el registro sencillo del objeto digital

dc.contributor.author	Arguedas Cortés, Donald
dc.contributor.author	Romero Zuñiga, Alex Patricio
dc.contributor.author	Enríquez, Ricardo
dc.contributor.author	MARTINEZ CASTAÑEDA, JOSE SIMON
dc.contributor.author	ORTEGA SANTANA, CESAR
dc.creator	Arguedas Cortés, Donald; 490703
dc.creator	Romero Zuñiga, Alex Patricio;x1256086
dc.creator	Enríquez, Ricardo;#0000-0001-5753-9363
dc.creator	MARTINEZ CASTAÑEDA, JOSE SIMON; 30838
dc.creator	ORTEGA SANTANA, CESAR; 282467
dc.date.accessioned	2016-03-16T17:17:19Z
dc.date.available	2016-03-16T17:17:19Z
dc.date.issued	2015
dc.identifier	http://www.redalyc.org/articulo.oa?id=44938603018
dc.identifier.uri	http://hdl.handle.net/20.500.11799/39268
dc.description	The infectious pancreatic necrosis (IPNV) is the causative agent of an acute illness well characterized in salmonids worldwide. Clinical signs and mortality rates are dependent on several factors such as the viral dose, the age of the fish, the water temperature, among others. An experimental study was conducted to measure the effect of temperature on the gene expression profile of IFN-1(α), STAT-1 and Mx-1 in rainbow trout fry, exposed to IPNV. Fry (n=198) were exposed at 8, 12 and 16°C, and samples were taken for 21 days to determine the virus titer and gene expression. In the first 11 days the greatest viral titer was recorded at 8°C compared with the values obtained at 12 and 16°C. At 8°C, there was a significant increase on day 4 of mRNA Mx-1 (t-test, p<0.05), time in which the viral titer began to decrease. Furthermore, as the viral titer increased, STAT-1 and Mx-1 (r=0.91) and (r=0.96) increased, respectively. The animals were able to recover from day 4 from some of the symptoms of IPN. Clinical disease was developed only in fish exposed to 12°C and all died between days 6 and 14, despite the highly significant increase shown in the average expression level of Mx-1, compared with the values recorded at 8°C and 16°C (Tukey, p<0.0001). Additionally, the expression profiles of IFN-1(α) and STAT-1 decreased completely (~0.016) and (~0.020 times) on day 7. The highest expression level of IFN-1(α), occurred at 16°C (Tukey, p<0.0005). Fry exposed at 16°C were normal during the experiment. IFN-1(α) possibly generated a protector effect from day 2 when they showed a significant expression increase compared with the results at 8°C and 12°C (t-student, p<0.0001); however, STAT-1 was not significantly affected by temperature, although the highest average expression value was recorded at 16°C. Our research supports the expression of relevant anti-viral response genes as IFN-1(α), STAT-1 and Mx-1 are physiologically modulated by the water temperature, directly influencing the development of the IPN disease in rainbow trout. Rev. Biol. Trop. 63 (2): 559-569. Epub 2015 June 01.
dc.format	application/pdf
dc.language.iso	eng	es
dc.publisher	Universidad de Costa Rica
dc.relation	http://www.redalyc.org/revista.oa?id=449
dc.rights	Revista de Biología Tropical
dc.rights	openAccess	es
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0
dc.source	Revista de Biología Tropical (Costa Rica) Num.2 Vol.63
dc.subject	Biología	es
dc.subject	IPNV	es
dc.subject	temperature	es
dc.subject	rainbow trout	es
dc.subject	fry	es
dc.subject	ISGs	es
dc.subject	real-time PCR	es
dc.subject	mortality	es
dc.subject.classification	CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA
dc.title	Effect of temperature on the expression of IFN-1 (α), STAT-1 and Mx-1 genes in Oncorhynchus mykiss (Salmoniformes: Salmonidae) exposed with the virus of the infectious pancreatic necrosis (IPNV)	es
dc.type	Artículo	es
dc.provenance	Científica
dc.road	Dorada	es
dc.ambito	Internacional	es
dc.audience	students	es
dc.audience	researchers	es
dc.type.conacyt	article
dc.identificator	6

Ficheros en el objeto digital

Este ítem aparece en la(s) siguiente(s) colección(ones)

• Conacyt [10019]
  
• Artículos publicados en revistas arbitradas de Redalyc.org [4450]
  

Visualización del Documento

Mostrar el registro sencillo del objeto digital