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dc.contributor.author	MALDONADO MAGAÑA, AMALIA
dc.contributor.author	OROZCO VILLAFUERTE, JUAN
dc.contributor.author	Buendía González, Leticia
dc.contributor.author	ESTRADA ZUÑIGA, MARIA ELENA
dc.contributor.author	BERNABE ANTONIO, ANTONIO
dc.contributor.author	CRUZ SOSA, FRANCISCO
dc.creator	MALDONADO MAGAÑA, AMALIA; 228818
dc.creator	OROZCO VILLAFUERTE, JUAN; 84355
dc.creator	Buendía González, Leticia; 42637
dc.creator	ESTRADA ZUÑIGA, MARIA ELENA; 164004
dc.creator	BERNABE ANTONIO, ANTONIO; 99963
dc.creator	CRUZ SOSA, FRANCISCO; 120198
dc.date.accessioned	2016-03-16T17:17:59Z
dc.date.available	2016-03-16T17:17:59Z
dc.date.issued	2013
dc.identifier	http://www.redalyc.org/articulo.oa?id=62029966011
dc.identifier.uri	http://hdl.handle.net/20.500.11799/39507
dc.description	In vitro studies indicate that Prosopis laevigata can be considered a potential hyperaccumulator of lead. Likewise, lead uptake has been related to protein transporters for zinc. In this work presents a protocol for the establishment of cell suspension culture to determine the effect of zinc on the uptake and accumulation of lead. A bioassay with Pb2+/Zn2+ (0.0, 0.5, and 1.0 mM) was carried out on cell suspension cultures derived from callus induced in half-strength Murashige and Skoog (MS) medium added with 6.8 pM 2,4-dichlorophenoxyacetic acid (2,4-D) with 4.5 pM kinetin (KIN). Cells showed significant tolerance to growth (GR>60%) at all concentrations and combinations of Pb and Zn (0.0, 0.5 and 1.0 mM). When the Pb with or without Zn were added to the culture medium, the cells showed the highest accumulation efficiency for non-essential (lead) metal over essential (zinc) metal (BF values for Pb » BF values for Zn; 2-33 times). Scanning electron micrographs evidenced the accumulation of Pb in the cells walls. These results provide insights about the tolerance and accumulation mechanisms of Pb occurring in P. laevigata.
dc.format	application/pdf
dc.language.iso	spa	es
dc.publisher	Universidad Autónoma Metropolitana Unidad Iztapalapa
dc.relation	http://www.redalyc.org/revista.oa?id=620
dc.rights	Revista Mexicana de Ingeniería Química
dc.rights	openAccess	es
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0
dc.source	Revista Mexicana de Ingeniería Química (México) Num.3 Vol.12
dc.subject	Ingeniería	es
dc.subject	Prosopis laevigata	es
dc.subject	lead	es
dc.subject	zinc	es
dc.subject	competitive transport	es
dc.subject	bioaccumulation	es
dc.subject	phytoremediation	es
dc.subject.classification	INGENIERÍA Y TECNOLOGÍA
dc.title	Establishment of cell suspension cultures of prosopis laevigata (humb. & bonpl. ex willd) m.c. johnst to determine the effect of zinc on the uptake and accumulation of lead	es
dc.type	Artículo	es
dc.provenance	Científica	es
dc.road	Dorada	es
dc.ambito	Internacional	es
dc.audience	students	es
dc.audience	researchers	es
dc.type.conacyt	article
dc.identificator	7

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