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dc.contributor FERRO-FLORES, GUILLERMINA
dc.contributor Ramírez, Flor de Maria
dc.contributor Ocampo-García, Blanca E.
dc.contributor Santos-Cuevas, Clara L.
dc.contributor Aranda-Lara, Liliana
dc.contributor azorin-vega, erika patricia
dc.contributor Morales-Avila, Enrique
dc.contributor Isaac-Olive, Keila
dc.contributor.author Mendoza-Nava, Héctor Javier
dc.date.accessioned 2017-03-10T15:42:39Z
dc.date.available 2017-03-10T15:42:39Z
dc.date.issued 29-06-16
dc.identifier.issn 1687-4110
dc.identifier.uri http://hdl.handle.net/20.500.11799/65730
dc.description.abstract 177Lu-labeled nanoparticles conjugated to biomolecules have been proposed as a new class of theranostic radiopharmaceuticals. The aim of this research was to synthesize 177Lu-dendrimer(PAMAM-G4)-folate-bombesin with gold nanoparticles (AuNPs) in the dendritic cavity and to evaluate the radiopharmaceutical potential for targeted radiotherapy and the simultaneous detection of folate receptors (FRs) and gastrin-releasing peptide receptors (GRPRs) overexpressed in breast cancer cells. p-SCN-Benzyl-DOTA was conjugated in aqueous-basic medium to the dendrimer.The carboxylate groups of Lys1Lys3(DOTA)-bombesin and folic acid were activatedwithHATUand also conjugated to the dendrimer.The conjugate was mixed with 1%HAuCl4 followed by the addition of NaBH4 and purified by ultrafiltration. Elemental analysis (EDS), particle size distribution (DLS), TEM analysis, UV-Vis, and infrared and fluorescence spectroscopies were performed. The conjugate was radiolabeled using 177LuCl3 or 68GaCl3 and analyzed by radio-HPLC. Studies confirmed the dendrimer functionalization with high radiochemical purity (>95%). Fluorescence results demonstrated that the presence ofAuNPs in the dendritic cavity confers useful photophysical properties to the radiopharmaceutical for optical imaging. Preliminary binding studies in T47D breast cancer cells showed a specific cell uptake (41.15 ± 2.72%). 177Ludendrimer( AuNP)-folate-bombesin may be useful as an optical and nuclear imaging agent for breast tumors overexpressing GRPR and FRs, as well as for targeted radiotherapy. es
dc.description.sponsorship CONACYT-SEP-CB-2014-01-242443 International Atomic Energy Agency (Grant 18358) LaboratorioNacional de Investigaci´on yDesarrollo de Radiof´armacos, CONACyT es
dc.language.iso eng es
dc.rights openAccess es
dc.rights https://creativecommons.org/licenses/by/4.0/ es
dc.rights openAccess es
dc.rights https://creativecommons.org/licenses/by/4.0/ es
dc.subject Dendrimers es
dc.subject Gold nanoparticles
dc.subject Theranostic Radiopharmaceutical
dc.title 177Lu-Dendrimer Conjugated to Folate and Bombesin with Gold Nanoparticles in the Dendritic Cavity: A Potential Theranostic Radiopharmaceutical es
dc.type Artículo es
dc.provenance Científica es
dc.road Dorada es
dc.ambito Internacional es
dc.cve.CenCos 20201 es
dc.cve.progEstudios 746 es
dc.modalidad Artículo especializado para publicar en revista indizada es


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  • Título
  • 177Lu-Dendrimer Conjugated to Folate and Bombesin with Gold Nanoparticles in the Dendritic Cavity: A Potential Theranostic Radiopharmaceutical
  • Autor
  • Mendoza-Nava, Héctor Javier
  • Colaborador
  • FERRO-FLORES, GUILLERMINA
  • Ramírez, Flor de Maria
  • Ocampo-García, Blanca E.
  • Santos-Cuevas, Clara L.
  • Aranda-Lara, Liliana
  • azorin-vega, erika patricia
  • Morales-Avila, Enrique
  • Isaac-Olive, Keila
  • Fecha de publicación
  • 29-06-16
  • Tipo de documento
  • Artículo
  • Palabras clave
  • Dendrimers
  • Gold nanoparticles
  • Theranostic Radiopharmaceutical
  • Los documentos depositados en el Repositorio Institucional de la Universidad Autónoma del Estado de México se encuentran a disposición en Acceso Abierto bajo la licencia Creative Commons: Atribución-NoComercial-SinDerivar 4.0 Internacional (CC BY-NC-ND 4.0)

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