Síntesis y caracterización de nanopartículas de Au y Ag. Evaluación toxicológica en un modelo in vivo

Abstract

In recent years, sustainable nanotechnologies have been promoted reduce the generation of hazardous waste to the environment, but little is known toxicological negative impact of nanomaterials on living organisms and cellular interaction with it. The metal nanoparticles have a wide range of applications, from the viewpoint of biological and medical synthetic thanks to their properties which in turn are influenced by its characteristics such as morphology, size, reactivity, concentration, and means in which are. That's why it is important to evaluate these materials and their toxicological part based on the properties of gold nanoparticles (Au) and silver (Ag) and the environment in which they are, these systems may present some specific toxicity, affecting significantly to the organisms that acquire and store. Initially presents the comparison between two methods for synthesizing metal nanoparticles (Au and Ag) using the chemical synthesis of sodium borohydride (NaBH4), while for the biological synthesis used aqueous extract of Camellia sinensis, followed by monitoring by UV-Vis and characterization by transmission electron microscopy (TEM) and light scattering (DLS) was subsequently performed toxicological studies (acute and subchronic) comparing the fourth systems in an in vivo model, that in assessing whether nanomaterials such together with the dispersing medium have a specific toxicity. Finally we evaluated the most susceptible to the deposition of Au and Ag nanoparticles in histological sections of liver and kidney, with two routes and Histology Epifluorescence animal.