Resumen:
Eight molecular-characterized isolates of Ralstonia solanacearum from potato belonging to race 3 biovar 2, their
virulence were evaluated on potato cv. Lady Rosette, tomato cv. Strain B, eggplant cv. Balady and pepper cv.
Balady and showed high virulence on potato and tomato, and lower virulence on eggplant and pepper. A laboratory
study conducted to produce polyclonal antibodies against the potato brown rot bacterium; R. solanacearum
cells were generated in female New Zealand white rabbits. A modification were made on the technique of
indirect enzyme-linked immunosorbent assay (ELISA) to improve the sensitivity of detection, including antigenic
and sensitivity to R. solanacearum race 3 biovar 2 isolates. Determination of the optimum period to collect the
antiserum (including, polyclonal antibodies) showed that the best collection dates were at 14, 3 and 7 days, in
that order.
Descripción:
The efficiency of the antiserum was compared among 42 isolates that cause potato brown rot disease;
our polyclonal antiserum (14 days) reacted positively with all tested isolates at a dilution of 1:6.4×103. Data
indicated the different reactions of eight R. solanacearum isolates at various dilutions (1:1.6×103 to
1:5.12×106) at 14 days against polyclonal antiserumat a concentration of approximately 1×108 CFU/mL and
we found the lowest detection level by the indirect ELISA technique was 106 CFU/mL. Finally we recommended
the reasonable sensitivity results of the ELISA technique to detect the bacterial pathogen given than the cost of
this technique if much lower than that of other expensive molecular techniques.