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dc.contributor.author Behiry, Said I.
dc.contributor.author Mohamed, Abeer A.
dc.contributor.author Younes, Hosny A.
dc.contributor.author Salem, Mohamed Z.M.
dc.date.accessioned 2018-02-08T23:20:16Z
dc.date.available 2018-02-08T23:20:16Z
dc.date.issued 2017-12-20
dc.identifier.issn 0882-4010
dc.identifier.uri http://hdl.handle.net/20.500.11799/68492
dc.description The efficiency of the antiserum was compared among 42 isolates that cause potato brown rot disease; our polyclonal antiserum (14 days) reacted positively with all tested isolates at a dilution of 1:6.4×103. Data indicated the different reactions of eight R. solanacearum isolates at various dilutions (1:1.6×103 to 1:5.12×106) at 14 days against polyclonal antiserumat a concentration of approximately 1×108 CFU/mL and we found the lowest detection level by the indirect ELISA technique was 106 CFU/mL. Finally we recommended the reasonable sensitivity results of the ELISA technique to detect the bacterial pathogen given than the cost of this technique if much lower than that of other expensive molecular techniques. es
dc.description.abstract Eight molecular-characterized isolates of Ralstonia solanacearum from potato belonging to race 3 biovar 2, their virulence were evaluated on potato cv. Lady Rosette, tomato cv. Strain B, eggplant cv. Balady and pepper cv. Balady and showed high virulence on potato and tomato, and lower virulence on eggplant and pepper. A laboratory study conducted to produce polyclonal antibodies against the potato brown rot bacterium; R. solanacearum cells were generated in female New Zealand white rabbits. A modification were made on the technique of indirect enzyme-linked immunosorbent assay (ELISA) to improve the sensitivity of detection, including antigenic and sensitivity to R. solanacearum race 3 biovar 2 isolates. Determination of the optimum period to collect the antiserum (including, polyclonal antibodies) showed that the best collection dates were at 14, 3 and 7 days, in that order. es
dc.language.iso eng es
dc.publisher ELSEVIR es
dc.relation.ispartofseries VOL;114
dc.rights embargoedAccess es
dc.rights https://creativecommons.org/licenses/by-sa/4.0/ es
dc.rights embargoedAccess es
dc.rights https://creativecommons.org/licenses/by-sa/4.0/ es
dc.subject Antigenic es
dc.subject Ralstonia solanacearum es
dc.subject Virulence es
dc.subject Polyclonal antibodies es
dc.subject Antiserum es
dc.title Antigenic and pathogenicity activities of Ralstonia solanacearum race 3 biovar 2 molecularly identified and detected by indirect ELISA using polyclonal antibodies generated in rabbits es
dc.type Artículo es
dc.provenance Científica es
dc.road Dorada es
dc.organismo Medicina Veterinaria y Zootecnia es
dc.ambito Internacional es


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  • Título
  • Antigenic and pathogenicity activities of Ralstonia solanacearum race 3 biovar 2 molecularly identified and detected by indirect ELISA using polyclonal antibodies generated in rabbits
  • Autor
  • Behiry, Said I.
  • Mohamed, Abeer A.
  • Younes, Hosny A.
  • Salem, Mohamed Z.M.
  • Fecha de publicación
  • 2017-12-20
  • Editor
  • ELSEVIR
  • Tipo de documento
  • Artículo
  • Palabras clave
  • Antigenic
  • Ralstonia solanacearum
  • Virulence
  • Polyclonal antibodies
  • Antiserum
  • Los documentos depositados en el Repositorio Institucional de la Universidad Autónoma del Estado de México se encuentran a disposición en Acceso Abierto bajo la licencia Creative Commons: Atribución-NoComercial-SinDerivar 4.0 Internacional (CC BY-NC-ND 4.0)

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